Analysis of rate heterogeneity

Section author: Gavin Huttley

A simple example for analyses involving rate heterogeneity among sites. In this case we will simulate an alignment with two rate categories and then try to recover the rates from the alignment.

from cogent3.evolve.substitution_model import TimeReversibleNucleotide
from cogent3 import load_tree

Make an alignment with equal split between rates 0.6 and 0.2, and then concatenate them to create a new alignment.

model = TimeReversibleNucleotide(equal_motif_probs=True)
tree = load_tree("data/test.tree")
lf = model.make_likelihood_function(tree)
lf.set_param_rule("length", value=0.6, is_constant=True)
aln1 = lf.simulate_alignment(sequence_length=10000)
lf.set_param_rule("length", value=0.2, is_constant=True)
aln2 = lf.simulate_alignment(sequence_length=10000)
aln3 = aln1 + aln2

Start from scratch, optimising only rates and the rate probability ratio.

model = TimeReversibleNucleotide(
    equal_motif_probs=True, ordered_param="rate", distribution="free"
)
lf = model.make_likelihood_function(tree, bins=2, digits=2, space=3)
lf.set_alignment(aln3)
lf.optimise(local=True, max_restarts=2, show_progress=False)

We want to know the bin probabilities and the posterior probabilities.

bprobs = [t for t in lf.get_statistics() if "bin" in t.title][0]
bprobs
bin params
binbprobsrate
bin00.530.50
bin10.471.56

2 rows x 3 columns

We’ll now use a gamma distribution on the sample alignment, specifying the number of bins as 4. We specify that the bins have equal density using the lf.set_param_rule('bprobs', is_constant=True) command.

model = TimeReversibleNucleotide(
    equal_motif_probs=True, ordered_param="rate", distribution="gamma"
)
lf = model.make_likelihood_function(tree, bins=4)
lf.set_param_rule("bprobs", is_constant=True)
lf.set_alignment(aln3)
lf.optimise(local=True, max_restarts=2, show_progress=False)